Cell-substrate impedance fluctuations of single amoeboid cells encode cell-shape and adhesion dynamics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26871108.
- Also identified by DOI 10.1103/PhysRevE.93.012414.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We show systematic electrical impedance measurements of single motile cells on microelectrodes. Wild-type cells and mutant strains were studied that differ in their cell-substrate adhesion strength. We recorded the projected cell area by time-lapse microscopy and observed irregular oscillations of the cell shape. These oscillations were correlated with long-term variations in the impedance signal. Superposed to these long-term trends, we observed fluctuations in the impedance signal. Their magnitude clearly correlated with the adhesion strength, suggesting that strongly adherent cells display more dynamic cell-substrate interactions.
Medical subject headings
- Cell Adhesion
- Dictyostelium
- Microelectrodes