Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale.
basic_science · Level V
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- Record sourced from PubMed, PMID 26878382.
- Also identified by DOI 10.1038/nmeth.3769 and PMC identifier 4814340.
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Abstract
Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.
Medical subject headings
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence
- Molecular Imaging
- RNA, Transfer
- Ribosomes