Calcium imaging of sleep-wake related neuronal activity in the dorsal pons.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26911837.
- Also identified by DOI 10.1038/ncomms10763 and PMC identifier 4773416.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The dorsal pons has long been implicated in the generation of rapid eye movement (REM) sleep, but the underlying circuit mechanisms remain poorly understood. Using cell-type-specific microendoscopic Ca(2+) imaging in and near the laterodorsal tegmental nucleus, we found that many glutamatergic neurons are maximally active during REM sleep (REM-max), while the majority of GABAergic neurons are maximally active during wakefulness (wake-max). Furthermore, the activity of glutamatergic neurons exhibits a medio-lateral spatial gradient, with medially located neurons more selectively active during REM sleep.
Medical subject headings
- Calcium
- GABAergic Neurons
- Pontine Tegmentum
- Sleep, REM
- Wakefulness