Super-Resolved Traction Force Microscopy (STFM).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26923775.
- Also identified by DOI 10.1021/acs.nanolett.6b00273 and PMC identifier 4898862.
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Abstract
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
Medical subject headings
- Computer Simulation
- Microscopy, Scanning Probe
- Models, Theoretical
- Traction