T cell fate and clonality inference from single-cell transcriptomes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26950746.
- Also identified by DOI 10.1038/nmeth.3800 and PMC identifier 4835021.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We developed TraCeR, a computational method to reconstruct full-length, paired T cell receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links T cell specificity with functional response by revealing clonal relationships between cells alongside their transcriptional profiles. We found that T cell clonotypes in a mouse Salmonella infection model span early activated CD4(+) T cells as well as mature effector and memory cells.
Medical subject headings
- CD4-Positive T-Lymphocytes
- High-Throughput Nucleotide Sequencing
- Receptors, Antigen, T-Cell
- Salmonella Infections, Animal
- Single-Cell Analysis
- Software
- Transcriptome