Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26956772.
- Also identified by DOI 10.1038/ncomms10940 and PMC identifier 4786873.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity.
Medical subject headings
- Antifungal Agents
- Aspergillosis
- Aspergillus fumigatus
- Boron Compounds
- Diagnostic Imaging
- Fluorescent Dyes
- Peptides