Multiplexed single-molecule force spectroscopy using a centrifuge.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 26984516.
- Also identified by DOI 10.1038/ncomms11026 and PMC identifier 4800429.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present a miniature centrifuge force microscope (CFM) that repurposes a benchtop centrifuge for high-throughput single-molecule experiments with high-resolution particle tracking, a large force range, temperature control and simple push-button operation. Incorporating DNA nanoswitches to enable repeated interrogation by force of single molecular pairs, we demonstrate increased throughput, reliability and the ability to characterize population heterogeneity. We perform spatiotemporally multiplexed experiments to collect 1,863 bond rupture statistics from 538 traceable molecular pairs in a single experiment, and show that 2 populations of DNA zippers can be distinguished using per-molecule statistics to reduce noise.
Medical subject headings
- Centrifugation
- Microscopy, Atomic Force
- Spectrum Analysis