Super-Resolution Imaging of Molecular Emission Spectra and Single Molecule Spectral Fluctuations.
Where this comes from
- Record sourced from PubMed, PMID 27002724.
- Also identified by DOI 10.1371/journal.pone.0147506 and PMC identifier 4803349.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Localization microscopy can image nanoscale cellular details. To address biological questions, the ability to distinguish multiple molecular species simultaneously is invaluable. Here, we present a new version of fluorescence photoactivation localization microscopy (FPALM) which detects the emission spectrum of each localized molecule, and can quantify changes in emission spectrum of individual molecules over time. This information can allow for a dramatic increase in the number of different species simultaneously imaged in a sample, and can create super-resolution maps showing how single molecule emission spectra vary with position and time in a sample.
Medical subject headings
- Microscopy