Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27004937.
- Also identified by DOI 10.1038/ncomms11088 and PMC identifier 4814582.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
Medical subject headings
- Caenorhabditis elegans
- Embryo, Nonmammalian
- Imaging, Three-Dimensional
- Microscopy
- Zebrafish