Quantitative super-resolution imaging with qPAINT.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27018580.
- Also identified by DOI 10.1038/nmeth.3804 and PMC identifier 4941813.
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Abstract
Counting molecules in complexes is challenging, even with super-resolution microscopy. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantitative points accumulation in nanoscale topography (qPAINT), works independently of dye photophysics for robust counting with high precision and accuracy over a wide dynamic range. qPAINT was benchmarked on DNA nanostructures and demonstrated for cellular applications by quantifying proteins in situ and the number of single-molecule FISH probes bound to an mRNA target.
Medical subject headings
- DNA
- Image Enhancement
- In Situ Hybridization, Fluorescence
- Microscopy, Fluorescence