Cas9 Functionally Opens Chromatin.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27031353.
- Also identified by DOI 10.1371/journal.pone.0152683 and PMC identifier 4816323.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
Medical subject headings
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Chromatin
- Receptors, Retinoic Acid
- Transcriptional Activation