Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27031845.
- Also identified by DOI 10.1371/journal.pone.0152016 and PMC identifier 4816457.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.
Medical subject headings
- Adenosine Triphosphate
- Fibroblasts
- Glycolysis
- Myoblasts