Near-optimal probabilistic RNA-seq quantification.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27043002.
- Also identified by DOI 10.1038/nbt.3519.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We present kallisto, an RNA-seq quantification program that is two orders of magnitude faster than previous approaches and achieves similar accuracy. Kallisto pseudoaligns reads to a reference, producing a list of transcripts that are compatible with each read while avoiding alignment of individual bases. We use kallisto to analyze 30 million unaligned paired-end RNA-seq reads in <10 min on a standard laptop computer. This removes a major computational bottleneck in RNA-seq analysis.
Medical subject headings
- Algorithms
- High-Throughput Nucleotide Sequencing
- Models, Statistical
- RNA
- Sequence Analysis, RNA
- Software