Expansion microscopy with conventional antibodies and fluorescent proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27064647.
- Also identified by DOI 10.1038/nmeth.3833 and PMC identifier 4929147.
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Abstract
Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.
Medical subject headings
- Antibodies, Monoclonal
- Image Enhancement
- Luminescent Proteins
- Microscopy, Confocal
- Microscopy, Fluorescence