siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27088314.
- Also identified by DOI 10.1038/nmeth.3836.
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Abstract
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
Medical subject headings
- Image Enhancement
- Image Interpretation, Computer-Assisted
- Microscopy, Fluorescence
- Time-Lapse Imaging