Quantitative Microplate Assay for Real-Time Nuclease Kinetics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27101307.
- Also identified by DOI 10.1371/journal.pone.0154099 and PMC identifier 4839650.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.
Medical subject headings
- Endonucleases