Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27104343.
- Also identified by DOI 10.1371/journal.pone.0154209 and PMC identifier 4841507.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.
Medical subject headings
- Brucellosis, Bovine
- Chorioallantoic Membrane
- Proteome
- Proteomics