irCLIP platform for efficient characterization of protein-RNA interactions.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27111506.
- Also identified by DOI 10.1038/nmeth.3840 and PMC identifier 5477425.
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Abstract
The complexity of transcriptome-wide protein-RNA interaction networks is incompletely understood. While emerging studies are greatly expanding the known universe of RNA-binding proteins, methods for the discovery and characterization of protein-RNA interactions remain resource intensive and technically challenging. Here we introduce a UV-C crosslinking and immunoprecipitation platform, irCLIP, which provides an ultraefficient, fast, and nonisotopic method for the detection of protein-RNA interactions using far less material than standard protocols.
Medical subject headings
- Immunoprecipitation
- RNA-Binding Proteins
- Ultraviolet Rays