CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes.
basic_science · Level V
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- Record sourced from PubMed, PMID 27111720.
- Also identified by DOI 10.1038/nbt.3536.
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Abstract
High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-interference (CRISPRi), with a traditional short hairpin RNA (shRNA)-based system for performing lethality screens. We find that the CRISPR technology performed best, with low noise, minimal off-target effects and consistent activity across reagents.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Gene Expression Profiling
- Gene Knockout Techniques
- Genetic Markers
- Genetic Testing
- RNA, Small Interfering