An unbiased metric of antiproliferative drug effect in vitro.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27135974.
- Also identified by DOI 10.1038/nmeth.3852 and PMC identifier 4887341.
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Abstract
In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current metrics of antiproliferative small molecule effect suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.
Medical subject headings
- Cell Proliferation
- Drug Discovery
- Models, Theoretical
- Molecular Biology
- Small Molecule Libraries