Aggregation of AcMNPV LEF-10 and Its Impact on Viral Late Gene Expression.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27152613.
- Also identified by DOI 10.1371/journal.pone.0154835 and PMC identifier 4859499.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) late expression factor gene lef-10 has been identified to be required for viral late gene expression by transient expression assay. Our previous work has shown that the gene product LEF-10 can form very stable high-molecular-weight complexes, but the structure and function of the protein remain unknown. In this study, we demonstrated that LEF-10 was essential for the replication of AcMNPV, and its truncated fragment containing amino acid residues 1 to 48 were sufficient to support the virus survival. Our data also suggested that the LEF-10 could spontaneously aggregate to form punctate spots in virus infected Sf9 cells at low frequency, and the aggregation of the protein could be induced by LEF-10 over-expression. When the protein aggregated to form punctate spots, soluble LEF-10 proteins were depleted and this could result in the down-regulation of viral late gene expression.
Medical subject headings
- Gene Expression Regulation, Viral
- Genes, Viral
- Insect Proteins
- Nucleopolyhedroviruses