Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27159373.
- Also identified by DOI 10.1038/nbt.3567 and PMC identifier 4900911.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.
Medical subject headings
- CRISPR-Associated Proteins
- Clustered Regularly Interspaced Short Palindromic Repeats
- Genes, Essential
- High-Throughput Nucleotide Sequencing
- RNA, Small Interfering