DADA2: High-resolution sample inference from Illumina amplicon data.
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- Record sourced from PubMed, PMID 27214047.
- Also identified by DOI 10.1038/nmeth.3869 and PMC identifier 4927377.
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Abstract
We present the open-source software package DADA2 for modeling and correcting Illumina-sequenced amplicon errors (https://github.com/benjjneb/dada2). DADA2 infers sample sequences exactly and resolves differences of as little as 1 nucleotide. In several mock communities, DADA2 identified more real variants and output fewer spurious sequences than other methods. We applied DADA2 to vaginal samples from a cohort of pregnant women, revealing a diversity of previously undetected Lactobacillus crispatus variants.
Medical subject headings
- Computational Biology
- High-Throughput Nucleotide Sequencing
- Lactobacillus
- Microbiota
- Sequence Analysis, DNA
- Software