Comparison of Cas9 activators in multiple species.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 27214048.
- Also identified by DOI 10.1038/nmeth.3871 and PMC identifier 4927356.
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Abstract
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.
Medical subject headings
- CRISPR-Associated Proteins
- Drosophila melanogaster
- Trans-Activators
- Transcription Factors