Clear Plaque Mutants of Lactococcal Phage TP901-1.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27258092.
- Also identified by DOI 10.1371/journal.pone.0155233 and PMC identifier 4892519.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We report a method for obtaining turbid plaques of the lactococcal bacteriophage TP901-1 and its derivative TP901-BC1034. We have further used the method to isolate clear plaque mutants of this phage. Analysis of 8 such mutants that were unable to lysogenize the host included whole genome resequencing. Four of the mutants had different mutations in structural genes with no relation to the genetic switch. However all 8 mutants had a mutation in the cI repressor gene region. Three of these were located in the promoter and Shine-Dalgarno sequences and five in the N-terminal part of the encoded CI protein involved in the DNA binding. The conclusion is that cI is the only gene involved in clear plaque formation i.e. the CI protein is the determining factor for the lysogenic pathway and its maintenance in the lactococcal phage TP901-1.
Medical subject headings
- Bacteriophages
- DNA, Viral
- Lactococcus
- Mutation
- Viral Proteins