Dense transcript profiling in single cells by image correlation decoding.
basic_science · Level V
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- Record sourced from PubMed, PMID 27271198.
- Also identified by DOI 10.1038/nmeth.3895 and PMC identifier 4965285.
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Abstract
Sequential barcoded fluorescent in situ hybridization (seqFISH) allows large numbers of molecular species to be accurately detected in single cells, but multiplexing is limited by the density of barcoded objects. We present correlation FISH (corrFISH), a method to resolve dense temporal barcodes in sequential hybridization experiments. Using corrFISH, we quantified highly expressed ribosomal protein genes in single cultured cells and mouse thymus sections, revealing cell-type-specific gene expression.
Medical subject headings
- Dietary Proteins
- Gene Expression Profiling
- In Situ Hybridization, Fluorescence
- RNA, Messenger
- Single-Cell Analysis