Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27347754.
- Also identified by DOI 10.1038/nbt.3594 and PMC identifier 4980167.
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Abstract
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.
Medical subject headings
- Brain Mapping
- Microscopy, Fluorescence, Multiphoton
- Neurons
- Visual Cortex
- Visual Perception
- Voltage-Sensitive Dye Imaging