Envelope-specific B-cell populations in African green monkeys chronically infected with simian immunodeficiency virus.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27381634.
- Also identified by DOI 10.1038/ncomms12131 and PMC identifier 4935802.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
African green monkeys (AGMs) are natural primate hosts of simian immunodeficiency virus (SIV). Interestingly, features of the envelope-specific antibody responses in SIV-infected AGMs are distinct from that of HIV-infected humans and SIV-infected rhesus monkeys, including gp120-focused responses and rapid development of autologous neutralization. Yet, the lack of genetic tools to evaluate B-cell lineages hinders potential use of this unique non-human primate model for HIV vaccine development. Here we define features of the AGM Ig loci and compare the proportion of Env-specific memory B-cell populations to that of HIV-infected humans and SIV-infected rhesus monkeys. AGMs appear to have a higher proportion of Env-specific memory B cells that are mainly gp120 directed. Furthermore, AGM gp120-specific monoclonal antibodies display robust antibody-dependent cellular cytotoxicity and CD4-dependent virion capture activity. Our results support the use of AGMs to model induction of functional gp120-specific antibodies by HIV vaccine strategies.
Medical subject headings
- Antibodies, Neutralizing
- Antibodies, Viral
- B-Lymphocytes
- Immunoglobulins
- Simian Acquired Immunodeficiency Syndrome
- Simian Immunodeficiency Virus