HnRNP Q Has a Suppressive Role in the Translation of Mouse Cryptochrome1.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27392095.
- Also identified by DOI 10.1371/journal.pone.0159018 and PMC identifier 4938544.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Precise regulation of gene expression is especially important for circadian timekeeping which is maintained by the proper oscillation of the mRNA and protein of clock genes and clock-controlled genes. As a main component of the core negative arm feedback loops in the circadian clock, the Cry1 gene contributes to the maintenance of behavioral and molecular rhythmicity. Despite the central role of Cry1, the molecular mechanisms regulating expression levels of Cry1 mRNA and protein are not well defined. In particular, the post-transcriptional regulation of Cry1 mRNA fate decisions is unclear. Here, we demonstrate that hnRNP Q binds to mCry1 mRNA via the 5'UTR. Furthermore, hnRNP Q inhibits the translation of mCry1 mRNA, leading to altered rhythmicity in the mCRY1 protein profile.
Medical subject headings
- 5' Untranslated Regions
- Cryptochromes
- Gene Expression Regulation
- Heterogeneous-Nuclear Ribonucleoproteins
- Protein Biosynthesis