RNA polymerase I-Rrn3 complex at 4.8 Å resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27418309.
- Also identified by DOI 10.1038/ncomms12129 and PMC identifier 4947163.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Transcription of ribosomal DNA by RNA polymerase I (Pol I) requires the initiation factor Rrn3. Here we report the cryo-EM structure of the Pol I-Rrn3 complex at 4.8 Å resolution. The structure reveals how Rrn3 binding converts an inactive Pol I dimer into an initiation-competent monomeric complex and provides insights into the mechanisms of Pol I-specific initiation and regulation.
Medical subject headings
- Pol1 Transcription Initiation Complex Proteins
- RNA Polymerase I
- Saccharomyces cerevisiae Proteins