ntHash: recursive nucleotide hashing.

Mohamadi, Hamid; Chu, Justin; Vandervalk, Benjamin P; Birol, Inanc · Bioinformatics · 2016

basic_science · Level V

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Abstract

Hashing has been widely used for indexing, querying and rapid similarity search in many bioinformatics applications, including sequence alignment, genome and transcriptome assembly, k-mer counting and error correction. Hence, expediting hashing operations would have a substantial impact in the field, making bioinformatics applications faster and more efficient. We present ntHash, a hashing algorithm tuned for processing DNA/RNA sequences. It performs the best when calculating hash values for adjacent k-mers in an input sequence, operating an order of magnitude faster than the best performing alternatives in typical use cases. ntHash is available online at http://www.bcgsc.ca/platform/bioinfo/software/nthash and is free for academic use. hmohamadi@bcgsc.ca or ibirol@bcgsc.caSupplementary information: Supplementary data are available at Bioinformatics online.

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