LOVTRAP: an optogenetic system for photoinduced protein dissociation.
basic_science · Level V
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- Record sourced from PubMed, PMID 27427858.
- Also identified by DOI 10.1038/nmeth.3926 and PMC identifier 5137947.
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Abstract
LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.
Medical subject headings
- Light
- Optogenetics
- Phosphatidylinositol 3-Kinase
- Photoreceptors, Plant
- Proto-Oncogene Proteins c-vav
- rac1 GTP-Binding Protein