On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27447941.
- Also identified by DOI 10.1371/journal.pone.0159777 and PMC identifier 4957773.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We fabricated droplet-based microfluidic platform for copolymerizable microspheres with acrydite modified DNA probe. The copolymerizable 3-D polyacrylamide microspheres were successfully produced from microcontinuous-flow synthesis with on-channel solidification. DNA copolymerization activity, surface presentation and thermostability were assessed by using fluorescent labeled complementary probe. The binding performance was only visible on the surface area of oligo-microspheres. We show that the resulting oligo-microspheres can be directly integrated into a streamlined microsphere-PCR protocol for amplifying ssDNA. Our microspheres could be utilized as a potential material for ssDNA analysis such as DNA microarray and automatic DNA SELEX process.
Medical subject headings
- DNA, Single-Stranded
- Microfluidics
- Microspheres
- Oligonucleotide Probes