Virtual microfluidics for digital quantification and single-cell sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27479330.
- Also identified by DOI 10.1038/nmeth.3955 and PMC identifier 5007149.
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Abstract
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
Medical subject headings
- Genome, Bacterial
- High-Throughput Nucleotide Sequencing
- Microfluidics
- Nucleic Acid Amplification Techniques
- Sequence Analysis, DNA
- Single-Cell Analysis