Cellular resolution circuit mapping with temporal-focused excitation of soma-targeted channelrhodopsin.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27525487.
- Also identified by DOI 10.7554/eLife.14193 and PMC identifier 5001837.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We describe refinements in optogenetic methods for circuit mapping that enable measurements of functional synaptic connectivity with single-neuron resolution. By expanding a two-photon beam in the imaging plane using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s, and determine the presence or absence of synaptic connections with patch-clamp electrophysiological recording.
Medical subject headings
- Brain Mapping
- Nerve Net
- Optogenetics
- Patch-Clamp Techniques