Comparative Evaluation of Real-Time PCR Methods for Human Noroviruses in Wastewater and Human Stool.
Where this comes from
- Record sourced from PubMed, PMID 27525654.
- Also identified by DOI 10.1371/journal.pone.0160825 and PMC identifier 4985124.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Selecting the best quantitative PCR assay is essential to detect human norovirus genome effectively from clinical and environmental samples because no cell lines have been developed to propagate this virus. The real-time PCR methods for noroviruses GI (4 assays) and GII (3 assays) were evaluated using wastewater (n = 70) and norovirus-positive stool (n = 77) samples collected in Japan between 2012 and 2013. Standard quantitative PCR assays recommended by the U.S. Environmental Protection Agency, International Organization for Standardization, and Ministry of Health, Labour and Welfare, Japan, together with recently reported assays were included. Significant differences in positive rates and quantification cycles were observed by non-parametric analysis. The present study identifies the best assay for norovirus GI and GII to amplify norovirus genomes efficiently.
Medical subject headings
- Feces
- Norovirus
- Real-Time Polymerase Chain Reaction
- Wastewater