DSBCapture: in situ capture and sequencing of DNA breaks.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27525976.
- Also identified by DOI 10.1038/nmeth.3960 and PMC identifier 5045719.
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Abstract
Double-strand DNA breaks (DSBs) continuously arise and cause mutations and chromosomal rearrangements. Here, we present DSBCapture, a sequencing-based method that captures DSBs in situ and directly maps these at single-nucleotide resolution, enabling the study of DSB origin. DSBCapture shows substantially increased sensitivity and data yield compared with other methods. Using DSBCapture, we uncovered a striking relationship between DSBs and elevated transcription within nucleosome-depleted chromatin.
Medical subject headings
- DNA
- DNA Breaks, Double-Stranded
- High-Throughput Nucleotide Sequencing