Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27558844.
- Also identified by DOI 10.1038/ncomms12562 and PMC identifier 5007294.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs in vivo.
Medical subject headings
- Fluorescence Recovery After Photobleaching
- Membrane Proteins
- Nuclear Envelope
- Single Molecule Imaging