Vibrio natriegens as a fast-growing host for molecular biology.

Weinstock, Matthew T; Hesek, Eric D; Wilson, Christopher M; Gibson, Daniel G · Nat Methods · 2016

basic_science · Level V

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Abstract

A rapidly growing bacterial host would be desirable for a range of routine applications in molecular biology and biotechnology. The bacterium Vibrio natriegens has the fastest growth rate of any known organism, with a reported doubling time of <10 min. We report the development of genetic tools and methods to engineer V. natriegens and demonstrate the advantages of using these engineered strains in common biotech processes.

Medical subject headings