Diffusion pseudotime robustly reconstructs lineage branching.
basic_science · Level V
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- Record sourced from PubMed, PMID 27571553.
- Also identified by DOI 10.1038/nmeth.3971.
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Abstract
The temporal order of differentiating cells is intrinsically encoded in their single-cell expression profiles. We describe an efficient way to robustly estimate this order according to diffusion pseudotime (DPT), which measures transitions between cells using diffusion-like random walks. Our DPT software implementations make it possible to reconstruct the developmental progression of cells and identify transient or metastable states, branching decisions and differentiation endpoints.
Medical subject headings
- Cell Differentiation
- Cell Lineage
- High-Throughput Nucleotide Sequencing
- Models, Genetic
- Models, Statistical
- Single-Cell Analysis