Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27610567.
- Also identified by DOI 10.1016/j.cell.2016.08.050 and PMC identifier 5589196.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.
Medical subject headings
- Mediator Complex
- Models, Molecular
- RNA Polymerase II
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins