The 9aaTAD Transactivation Domains: From Gal4 to p53.
Where this comes from
- Record sourced from PubMed, PMID 27618436.
- Also identified by DOI 10.1371/journal.pone.0162842 and PMC identifier 5019370.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The family of the Nine amino acid Transactivation Domain, 9aaTAD family, comprises currently over 40 members. The 9aaTAD domains are universally recognized by the transcriptional machinery from yeast to man. We had identified the 9aaTAD domains in the p53, Msn2, Pdr1 and B42 activators by our prediction algorithm. In this study, their competence to activate transcription as small peptides was proven. Not surprisingly, we elicited immense 9aaTAD divergence in hundreds of identified orthologs and numerous examples of the 9aaTAD species' convergence. We found unforeseen similarity of the mammalian p53 with yeast Gal4 9aaTAD domains. Furthermore, we identified artificial 9aaTAD domains generated accidentally by others. From an evolutionary perspective, the observed easiness to generate 9aaTAD transactivation domains indicates the natural advantage for spontaneous generation of transcription factors from DNA binding precursors.
Medical subject headings
- DNA-Binding Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors
- Transcriptional Activation
- Tumor Suppressor Protein p53