HiChIP: efficient and sensitive analysis of protein-directed genome architecture.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27643841.
- Also identified by DOI 10.1038/nmeth.3999 and PMC identifier 5501173.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.
Medical subject headings
- Cell Cycle Proteins
- Chromatin
- Chromatin Immunoprecipitation
- Chromosomal Proteins, Non-Histone
- DNA
- Genomics