Enzyme-catalyzed expressed protein ligation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27669326.
- Also identified by DOI 10.1038/nmeth.4004 and PMC identifier 5088058.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of this method. Here we employ subtiligase variants to efficiently ligate Cys-free peptides to protein thioesters. Using this method, we have more accurately determined the effect of C-terminal phosphorylation on the tumor suppressor protein PTEN.
Medical subject headings
- PTEN Phosphohydrolase
- Peptide Fragments
- Peptide Synthases
- Recombinant Proteins
- Subtilisins