CO<sub>2</sub> fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27687501.
- Also identified by DOI 10.1038/ncomms12800 and PMC identifier 5056431.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Maximizing the conversion of biogenic carbon feedstocks into chemicals and fuels is essential for fermentation processes as feedstock costs and processing is commonly the greatest operating expense. Unfortunately, for most fermentations, over one-third of sugar carbon is lost to CO<sub>2</sub> due to the decarboxylation of pyruvate to acetyl-CoA and limitations in the reducing power of the bio-feedstock. Here we show that anaerobic, non-photosynthetic mixotrophy, defined as the concurrent utilization of organic (for example, sugars) and inorganic (for example, CO<sub>2</sub>) substrates in a single organism, can overcome these constraints to increase product yields and reduce overall CO<sub>2</sub> emissions. As a proof-of-concept, Clostridium ljungdahlii was engineered to produce acetone and achieved a mass yield 138% of the previous theoretical maximum using a high cell density continuous fermentation process. In addition, when enough reductant (that is, H<sub>2</sub>) is provided, the fermentation emits no CO<sub>2</sub>. Finally, we show that mixotrophy is a general trait among acetogens.