Plasmid-based one-pot saturation mutagenesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27723752.
- Also identified by DOI 10.1038/nmeth.4029 and PMC identifier 5666567.
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Abstract
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single- or multi-site saturation mutagenesis libraries.
Medical subject headings
- DNA
- Mutagenesis, Site-Directed
- Plasmids