Random-access scanning microscopy for 3D imaging in awake behaving animals.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27749836.
- Also identified by DOI 10.1038/nmeth.4033 and PMC identifier 5769813.
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Abstract
Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of neocortical pyramidal cells in awake behaving mice.
Medical subject headings
- Behavior, Animal
- Imaging, Three-Dimensional
- Microscopy, Fluorescence, Multiphoton
- Motor Activity
- Neurons
- Voltage-Sensitive Dye Imaging