Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27787919.
- Also identified by DOI 10.1002/adma.201603318.
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Abstract
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.
Medical subject headings
- Cell Culture Techniques
- Human Embryonic Stem Cells
- RNA Interference
- RNA, Small Interfering