Single-cell sequencing of the small-RNA transcriptome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27798564.
- Also identified by DOI 10.1038/nbt.3701.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Human Embryonic Stem Cells
- MicroRNAs
- Neoplasms, Experimental
- Sequence Analysis, RNA
- Transcriptome