Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 27798609.
- Also identified by DOI 10.1038/nmeth.4046 and PMC identifier 5322478.
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Abstract
We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.
Medical subject headings
- Biosensing Techniques
- Fluorescence Resonance Energy Transfer
- Fluorescent Dyes
- Green Fluorescent Proteins
- Luminescent Proteins
- Optical Imaging